SKILLS PORTAL

ព្រះរាជាណាចក្រកម្ពុជា · KINGDOM OF CAMBODIA

Cambodian Skills Delivery Model

SEAL National Course Catalogue · MLVT / National Training Board

Sector Frameworks

1,164

Qualifications

15,161

Competency Units

10,938

Elements

41,782

Learning Outcomes

2,822

Assessment Packages

Perform mycology assays

KHU-AGR-0060

Australian Equivalent

AHCARB805

Elements

Learning Outcomes

This unit of competency describes the skills and knowledge required to safely work within a laboratory environment, collect and identify wood decay fungi specimens from the field, prepare in-vitro cultures, and carry out primary experiments on fungi cultures. Work is performed using laboratory hygiene and safety procedures for sampling, sterilisation and cleaning of laboratory equipment and instruments. The unit applies to individuals with highly specialised advanced theoretical and technical knowledge for professional work and research in arboriculture. They exercise advanced cognitive, technical and communication skills and demonstrate complete autonomy, judgement and adaptability in research and analysis for complex problems. No licensing, legislative or certification requirements are to this unit at the time of publication.

Elements & Learning Outcomes

E1Research pathogenic and saprophytic wood decay fungi species

0060.E1.O1Investigate role of microbiological organisms on tree and forest health

0060.E1.O2Investigate the role and contribution of wood decay fungi to forest and tree health, and responses

0060.E1.O3Examine the taxonomy and evolutionary relationships of corticoid and polypore wood decay fungi

0060.E1.O4Investigate the role of fungal species on specific host trees

0060.E1.O5Analyse lifecycle, biology, ecology and effects of wood decay fungal species

0060.E1.O6Examine relationship between fungal species and tree defects and failures

0060.E1.O7Analyse biosecurity implications of pathogenic fungal species

0060.E1.O8Review state and federal biosecurity plans and procedures

E2Evaluate decay and identify fungi in trees

0060.E2.O1Observe and evaluate signs and symptoms of fungi causing decay in trees

0060.E2.O2Identify wood decaying fungi to generic level in field

0060.E2.O3Identify non-pathogenic fungi to generic level in field

0060.E2.O4Record location, size, and condition of wood decay fungi and mycoparasites of hollows and cavities in trees

0060.E2.O5Document details of environmental characteristics of fungal affected trees

0060.E2.O6Determine methods of dissemination and infection of fungi to other trees and vegetation

0060.E2.O7Sample wood decay fungi and mycoparasites from trees for in-vitro culture and identification

E3Prepare in-vitro media, cultures

0060.E3.O1Select, check and fit personal protective equipment and contamination prevention clothing according to workplace procedures

0060.E3.O2Decant and prepare standard laboratory chemicals and materials

0060.E3.O3Prepare selective media to isolate and culture mycology specimens

0060.E3.O4Prepare field samples ready for culturing on media

0060.E3.O5Excise specimens and apply to media

0060.E3.O6Maintain cultures and repeat excise procedures to isolate clean specimens

0060.E3.O7Prepare and maintain cultured samples for further testing

0060.E3.O8Update records and store securely according to chain of evidence protocols

E4Conduct laboratory identification and assays

0060.E4.O1Prepare microscope slides of isolated cultures

0060.E4.O2Examine and identify cultured fungal samples

0060.E4.O3Perform laboratory identification of wood decay fungi to generic level

0060.E4.O4Record digital images of identified fungi

0060.E4.O5Perform laboratory assay tests to evaluate fungal characteristics

0060.E4.O6Document experimental assay test results

0060.E4.O7Develop and maintain mycology culture collection and submit to government database and culture collections according to procedures

0060.E4.O8Prepare a diagnostic report on suspected emergency plant pest according to state and federal biosecurity procedures